Purpose: This study explored the perceptions of cervical cancer among female defectors from North Korea. Methods: A qualitative method using focus group interviews was employed. Thirteen participants who were older than 20 when they left North Korea were recruited through a church-based community in Seoul. Data were collected from 3 focus group interviews conducted in July 2021 after obtaining written consent from each participant. Each interview lasted for 40 to 50 minutes and was recorded. After verbatim transcription, the data were analyzed qualitatively using the conventional content analysis method. Results: The following four categories were identified, each with two to three subcategories: misconceptions about cervical cancer, barriers to knowledge acquisition, negative emotions toward the screening test, and expectations for cervical cancer education. Conclusion: North Korean defectors have been considered particularly vulnerable to cervical cancer, a major women’s health issue. Education and healthcare services need to be integrated into the services they receive after they enter South Korea.