This study aimed to compare the prosodic characteristics of North Korean defectors and South Koreans in three types of sentences (declarative, interrogative, and negative) in two reading tasks (short and dialogue) through acoustic analysis and auditory-perceptual evaluation. In addition, this study examined the relationship between the auditory-perceptual evaluation scores and self-assessment questionnaires on intonation for North Korean defectors. The participants were 15 North Korean defectors and 15 Korean speakers with standard Seoul accents. For statistical analysis, three-way mixed ANOVA and multivariate analysis were performed within the three types of sentences in the reading tasks through acoustic analysis and the Mann-Whitney U Test for auditory-perceptual evaluation. Pearson’s product-moment correlation coefficients were also used to identify the correlations between the results of the self-assessment questionnaire on intonation and the auditory-perceptual evaluation. The North Korean defectors were found to have a significantly lower pitch range and auditory-perceptual evaluation score than South Koreans in reading tasks. Moreover, there was a significant correlation between their auditory-perceptual evaluations and self-assessment questionnaires on intonation. The study findings suggest that North Korean defectors, who face many challenges with intonation, showed a tendency to think that their intonation differed from the standard Korean intonation and showed better auditory evaluation results for interrogative sentences.