Purpose: The purpose of this study was to investigate the knowledge and prevention attitudes of female North Korean refugees regarding cervical cancer and human papillomavirus(HPV) infection. Methods: A descriptive study was conducted. Data were collected from November 2015 to March 2016 and 130 female North Korean refugees participated. Data were analyzed using SPSS 21.0. Results: Score on knowledge and prevention attitudes regarding cervical cancer and HPV were 2.53 out of 8 points, 1.67 out of 17 points, and 28.90 out of 36 points. There were significant differences between entry duration, the number of children, previous cervical cancer tests, Precognition about HPV, preferred education method, and knowledge of cervical cancer. There were significant differences between previous cervical cancer tests, Precognition about HPV and knowledge of HPV. The correlation between knowledge and prevention attitudes regarding cervical cancer and HPV was significant positive. Conclusion: Female North Korean refugees’ knowledge level of cervical cancer and HPV is very low. However, their attitudes towards prevention was higher. These results imply a need for education, aimed at guiding North Korean female refugees, so as to enhance their knowledge regarding cervical cancer and HPV, and to promote their sexual health.